maa beads  (Bio-Rad)

Journal: BMC Microbiology

Article Title: Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells

doi: 10.1186/1471-2180-12-162

Figure Lengend Snippet: The attachment and infection of EV71 to RD cells are affected by sialic acid specific lectin treatment. Cells were preincubated with MAA ( maackia amurensis ) or SNA ( sambucus nigra ) followed by infection with EV71 MP4. The bound EV71 was analyzed by ELISA and real-time PCR, and the subsequent replication of EV71 in RD cells was detected by real-time PCR analysis. The binding of virus to RD cells treated with different concentrations of MAA was reduced by 19% and 45% measured by ELISA ( A ) and by 37% and 68% measured by real-time PCR ( C ). The replication of EV71 dropped 38% and 59% after MAA treatment measured by real-time PCR after 24 hours incubation ( E ). The virus binding of SNA treated cells reduced by 18% and 38% measured by ELISA ( B ), and by 28% and 45% measured by real-time PCR ( D ). The replication of EV71 dropped 30% and 58% after SNA treatment measured by RT-PCR after 24 hours incubation ( F ). **: P < 0.01; ***: P < 0.001 (two-tailed test). Each of the results was averaged from at least six independent assays.

Article Snippet: Then, membrane protein fractions were subjected to lectin affinity chromatography that was packaged with SNA and MAA agarose beads (EY Laboratories).

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Binding Assay, Incubation, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test

Journal: BMC Microbiology

Article Title: Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells

doi: 10.1186/1471-2180-12-162

Figure Lengend Snippet: The infection and replication of EV71 to RD cells are affected by lectin treatment investigated with EV71-GFP infection. Cells preincubated with or without MAA/SNA were infected with or without EV71-GFP. The cell number, CPE, and fluorescence intensity were observed by fluorescence microscope. After 48 hours, higher fluorescence intensity was found in untreated cells than neuraminidase pretreated cells.

Article Snippet: Then, membrane protein fractions were subjected to lectin affinity chromatography that was packaged with SNA and MAA agarose beads (EY Laboratories).

Techniques: Infection, Fluorescence, Microscopy

Journal: BMC Microbiology

Article Title: Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells

doi: 10.1186/1471-2180-12-162

Figure Lengend Snippet: The attachment and infection of EV71 4643 to SK-N-SH cells are affected by neuraminidase or sialic acid specific lectin treatment. SK-N-SH cells were pretreated with neuraminidase, MAA or SNA before infected with EV71 4643. ( A ) The copy number of EV71 dropped 44% and 59% in neuraminidase treated cells. ( B ) The copy number of EV71 reduced by 42% and 59% in MAA treated cells. ( C ) The copy number of EV71 decreased by 31% and 52% in SNA treated cells. **: P < 0.01; ***: P < 0.001 (two-tailed test). Each of the results was averaged from at least six independent assays.

Article Snippet: Then, membrane protein fractions were subjected to lectin affinity chromatography that was packaged with SNA and MAA agarose beads (EY Laboratories).

Techniques: Infection, Two Tailed Test

Journal: BMC Microbiology

Article Title: Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells

doi: 10.1186/1471-2180-12-162

Figure Lengend Snippet: Western blotting of glycoprotein fractions with anti-SCARB2 antibody. Lane 1: RD cell membrane extracts; Lane 2: MAA/SNA lectin affinity chromatography purified sialylated glycoproteins; Lane 3: desialylated glycoproteins; Lane 4: desialylated glycoproteins immunoprecipitated with EV71 MP4. All of the purified proteins were subjected to western blotting and stained by anti-SCARB2 antibody. SCARB2 could be observed in all of the fractions. Band in lane 3 was slightly shifted down after neuraminidase treatment. But, owing to non-reducing condition, band in lane 4 was slightly shifted up compared to band in lane 3.

Article Snippet: Then, membrane protein fractions were subjected to lectin affinity chromatography that was packaged with SNA and MAA agarose beads (EY Laboratories).

Techniques: Western Blot, Affinity Chromatography, Purification, Immunoprecipitation, Staining

Journal: British Journal of Cancer

Article Title: Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level

doi: 10.1038/bjc.2012.12

Figure Lengend Snippet: Melanoma cells isolated from peripheral blood. HMW-MAA + , CD45 − cells were isolated on immunomagnetic beads and stained with a mixture of MART-1 and gp100 antibodies. MART-1/gp100-positive cells stained brownish-red with aminoethylcarbazole. ( A ) Spiked MMG-1 cells isolated from the peripheral blood of a healthy individual. The small light-brown spheroid is an immunomagnetic bead. ( B ) CTC detected in a patient with melanoma. Scar bar: 5 μ m.

Article Snippet: Circulating tumour cells were isolated from the mononucleated cells using HMW-MAA-specific mAb-coated immunomagnetic beads (Dynabeads CELLection Pan Mouse IgG Kit; Invitrogen, Oslo, Norway) according to manufacturer's instructions with minor modifications.

Techniques: Isolation, Staining